Excalibur Field Trials

 In Horizon 2020

EXCALIBUR FIELD TRIALS – A TOOL TO ASSESS THE EFFECT OF MICROBIAL INOCULA TO SOIL BIODIVERSITY AND PLANT PERFORMANCE

Article by Eligio Malusá, Lidia Sas-Paszt, Małgorzata Tartanus

Instytut Ogrodnictwa (Research Institute of Horticulture – INHORT), Skierniewice, Poland.

 

The field trials planned in the project are playing a key role to achieve the project objective, which is to improve the knowledge on soil biodiversity dynamics in relation to the different agro-ecological factors to enhance the efficacy of biocontrol and biofertilization practices in horticultural farming. For this reason, they have been defined in relation to the climatic and soil characteristics present in the different countries of the project’s partners institutions for the three crops concerned: apple,

strawberry and tomato (Fig. 1-3). In particular, the different following factors, which are known to affect the efficacy of applied biofertilizers and biopesticides, have been considered in setting the trials:

  • Soil management (organic or integrated) – including the following different practices: fertilization (organic/inorganic), tillage (reduced/intensive), cover crops/mulching (yes/no), pre-planting inoculation (yes/no), soil-borne pathogens management (yes/no);
  • Canopy management – including the following treatments: foliar/soil application of biofertilizers/biopesticides, frost protection (yes/no);
  • Microbial-based products – including the following treatments: formulations (consortia or single strain), complex formulations (microbial inoculum +plant biostimulant or single product).

The number of microbial inocula or formulations planned to be tested in the different trials vary depending on the aspect to be addressed (i.e. plant protection in case of biopesticides or plant growth in case of biofertilizers) and the strains available. Furthermore, they depend on the different characteristics/needs of the three crops as well as of their production system (open field or protected crop).

A comprehensive analytical assessment of initial soil physico-chemical

-biological properties for all experimental sites is planned to be performed to obtain the largest and deepest possible assessment of the native biodiversity in the field hosting the trials. This will be followed by the monitoring of changes in these properties as well as by evaluating how these properties are affecting the inoculated strains during the trials.

To this aim, the soil mineralogical, textural and chemical composition is planned to be determined in association with direct pedological observations (profiles) using also innovative methods like proximal soil sensing (electromagnetic induction, gamma-ray spectroscopy). This work is expected to determine their main pedogenetic processes, soil characteristics and limitations (among them waterlogging susceptibility, nutrient status, compaction) which could strongly affect the success and the dynamics of inoculated microorganism. A particular focus in this respect is posed on nutrients content (macro and micro-elements) and the soil organic matter and its fractions.

The soil microbial diversity (bacteria, archaea, fungi, oomycetes and microbiome structure) will be assessed using both culturable-dependent procedures and DNA-based methods on both bulk and rhizospheric soils and the plant tissues. The latter will be analysed particularly to check the presence of mycorrhizal fungi in roots and their level of colonization.

Considering that macro, meso and microfauna populations (earthworms, arthropods, nematodes, protists) in and above the soil are affecting the persistence and dynamics of inoculated microorganisms (but are also affected by bio-inocula), we have planned to evaluate these biological elements in the trials. The data from soil samples will be used to calculate diversity indices, based on the concept that the number of groups morphologically well adapted to the soil is proportional to the soil quality.

Data obtained from the different analyses and trials (including classical diversity indices – e.g. Shannon’s index and other indexes – e.g. the QBS-ar index which better define the biological status of the agro-ecosystem) will be organized and elaborated to draw an overall framework of the initial biodiversity of soils and cropping systems under contrasting pedo-climatic conditions. Indeed, soil micro and macro organisms form complex interactions within specific ecological niches. By exploring these interactions, also with the use of multivariate network analyses, we believe that we will be able to understand the complexity of functional processes before any crop species is introduced in the field and during the growth of the crops treated with the selected inocula. With this knowledge, we plan to develop a decision supporting system that could help farmers and advisors in obtaining the best result from the biofertilizers and biopesticides applied in their fields.

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We had the possibility to use the public Puerto Rico transport only as soon as, in the form of a ferry, and if all the remainder works according to similar policies, I strongly recommend this auto than stressful Suggestion – it’s actually worth leasing an auto with a higher suspension. We did not know regarding it and we chose to get some tiny gingerbread as a result of the very best rate. We swiftly regretted this decision, since some edges of Puerto Rico are best explored with a 4×4 SUV. Caribbean, an island in between the Caribbean Sea and also the North Atlantic Ocean, eastern of the Dominican Republic, tactically situated on the Mona Strait – a crucial course to the Panama Canal; San Juan is among the largest and ideal natural harbors in the Caribbean; a lot of small rivers as well as high hills in the center of the nation make sure good irrigation of the nation; the south shore is reasonably completely dry; in the north, a productive strip of coastal plains. No one flies to Puerto Rico to see its cool federal government structures. You want enjoyable in the sunlight. Fortunately is that Flamenco Beach will measure up to your daydreams and after that some. Not just does it offer white sand as well as blue water that vibrates on the horizon, yet it likewise flaunts restrooms, lifeguards, food stands, volleyball webs and tourist stations where you can rent equipment for things like snorkeling and also diving. Puerto Rico’s economy is one of one of the most dynamically establishing in the Caribbean. The diversified sector has actually clearly outpaced agriculture as the main driving pressure of the economic situation and an income source. Considering that the 1950s, American business have actually been spending heavily on the island, urged by duty-free access to the US market and tax obligation breaks. The island has US base pay regulations. Sugar production lost its top setting in agriculture to milk and livestock manufacturing. Tourist is generally an important income source, it is estimated that Puerto Rico was gone to by almost 5 million people in 2004. 2001-03 saw a decline in financial development, mainly as a result of the US economic downturn; in 2004-05 there was a financial growth, as well as in 2006-07 it fell once again. Puerto Rico is one of the most dynamically creating Caribbean countries. The diversified industry has outdone agriculture as well as is currently the major income source. Considering that the 1950s, American firms have been buying the island, urged by the duty-free exchange with the USA and tax obligation rewards. The island goes through US minimum wage law. Sugar production decreased, as well as the milk industry and livestock production ended up being the major income. Tourist has actually been an important income source for many years. It is approximated that in 1999 Puerto Rico was gone to by 5 million visitors. The diagnosis for 2000 is excellent, supplied that the financial boom in the United States and also the growth patterns in tourism and also construction continue. Everyone desired an item of the New World, and everybody wanted to fight to get it. No place is this more apparent than the Castillo San Felipe del Morro. Formally called La Ruta del Lechón, the Pork Highway is precisely what it seems like: a three-mile stretch of roadway where every dining establishment specializes in pork. One of the most popular type of pork is lechón, a special meal in Puerto Rico that includes spit-roasting an entire pig with a mix of sauces and seasonings. Obtain your blood pumping with a trip to the Toro Verde Adventure Park in central Puerto Rico. It uses ziplines, rope training courses and also rock climbing obstacles that are ensured to make you pee your trousers a little when you understand simply what you have actually obtained into! The park’s main attraction is « The Monster, » which is provided in the Guinness Book of World Records as the longest zipline on the planet. With cobbled roads, active plazas as well as vibrant residences that stack together like building blocks of yellow, blue, eco-friendly, orange and also blue-green, Old San Juan is just one of one of the most lively places to see in Puerto Rico. This is why locations like Isla Culebrita exist. Isla Culebrita is an uninhabited island off the shore of eastern Puerto Rico. It’s only concerning a mile long, and also gain access to is limited to ferries and also water taxis. Museo de Las Americas There are numerous galleries in Puerto Rico, yet just a handful are in fact regarding Puerto Rico. The Museo de Las Americas is just one of these priceless places. It has artifacts, tokens and textiles from loads of indigenous groups that stayed in Latin America before it was conquered, and also it can take you on a multi-continental journey through history. If you like to consume, Mayaguez is the city for you. It’s residence to whatever from fine dining establishments to al fresco cafes with large, colorful umbrellas, so you’ll have lots of choices when your stomach begins growling. El Yunque National Forestis Treatment greater than 28,000 acres, the El Yunque National Park is a lavish, damp jungle where thick vegetation covers everything from wriggling serpents to croaking tree frogs. It’s the only tropical rainforest in Puerto Rico, as well as site visitors originate from throughout the Carribean to witness both its elegance and biodiversity. Found in the city of Cabo Rojo on the most southern tip of Puerto Rico, the Los Morrillos Lighthouse is one of those stunning areas that you can just find in the Caribbean. It’s set down on top of white sedimentary rock cliffs that are bordered by salt marshes as well as lagoons, and also the waves collision versus the cliffs with a physical violence that’s shocking but stunning. Cayo Santiago is a little stretch of land off the eastern coast of Puerto Rico.

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